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u one step qrt pcr probe 5 × master mix  (Vazyme Biotech Co)
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one step qrt pcr supermix kit  (BioChain Institute)
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superscript iii platinum sybr green one-step quantitative rt-pcr (qrt-pcr) kit  (Thermo Fisher)
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one step qrt pcr kit  (Thermo Fisher)
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hiscript ii q rt kit  (Vazyme Biotech Co)
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one step qrt pcr super mix kit  (tiangen biotech co)
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tiangen biotech co one step qrt pcr super mix kit
Diarrhea scores, fecal viral shedding, virus distribution and the VH:CD ratios in piglets infected with the PDCoV CHN/GX/1468B/2017 strain. (A) Diarrhea scores of 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. Clinical symptoms were monitored daily, and the severity of diarrhea for each piglet was scored using the following criteria: 0 = normal, 1 = fecal deformity, 2 = mild diarrhea, and 3 = watery diarrhea. (B) Fecal viral shedding in 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. PDCoV RNA shedding in the feces was quantitated daily from the four challenged piglets. (C) Virus distribution in 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. PDCoV RNA loads in tissues collected at 7 DPI were measured by using <t>qRT-PCR.</t> (D) The ratio of villous height to crypt depth (VH:CD) in small intestines from piglets inoculated with the PDCoV CHN/GX/1468B/2017 strain. Each point/bar represents the average values of four piglets, the numbers above the bar in graphs (B,C) represent the ratio of PDCoV positive piglets to the total in each group, and the asterisk indicates a statistically significant difference between the negative control and CHN/GX/1468B/2017 groups (*** p < 0.001).
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Diarrhea scores, fecal viral shedding, virus distribution and the VH:CD ratios in piglets infected with the PDCoV CHN/GX/1468B/2017 strain. (A) Diarrhea scores of 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. Clinical symptoms were monitored daily, and the severity of diarrhea for each piglet was scored using the following criteria: 0 = normal, 1 = fecal deformity, 2 = mild diarrhea, and 3 = watery diarrhea. (B) Fecal viral shedding in 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. PDCoV RNA shedding in the feces was quantitated daily from the four challenged piglets. (C) Virus distribution in 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. PDCoV RNA loads in tissues collected at 7 DPI were measured by using qRT-PCR. (D) The ratio of villous height to crypt depth (VH:CD) in small intestines from piglets inoculated with the PDCoV CHN/GX/1468B/2017 strain. Each point/bar represents the average values of four piglets, the numbers above the bar in graphs (B,C) represent the ratio of PDCoV positive piglets to the total in each group, and the asterisk indicates a statistically significant difference between the negative control and CHN/GX/1468B/2017 groups (*** p < 0.001).

Journal: Frontiers in Veterinary Science

Article Title: Genetic Characteristics and Pathogenicity of a Novel Porcine Deltacoronavirus Southeast Asia-Like Strain Found in China

doi: 10.3389/fvets.2021.701612

Figure Lengend Snippet: Diarrhea scores, fecal viral shedding, virus distribution and the VH:CD ratios in piglets infected with the PDCoV CHN/GX/1468B/2017 strain. (A) Diarrhea scores of 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. Clinical symptoms were monitored daily, and the severity of diarrhea for each piglet was scored using the following criteria: 0 = normal, 1 = fecal deformity, 2 = mild diarrhea, and 3 = watery diarrhea. (B) Fecal viral shedding in 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. PDCoV RNA shedding in the feces was quantitated daily from the four challenged piglets. (C) Virus distribution in 7-day-old piglets challenged with the PDCoV CHN/GX/1468B/2017 strain. PDCoV RNA loads in tissues collected at 7 DPI were measured by using qRT-PCR. (D) The ratio of villous height to crypt depth (VH:CD) in small intestines from piglets inoculated with the PDCoV CHN/GX/1468B/2017 strain. Each point/bar represents the average values of four piglets, the numbers above the bar in graphs (B,C) represent the ratio of PDCoV positive piglets to the total in each group, and the asterisk indicates a statistically significant difference between the negative control and CHN/GX/1468B/2017 groups (*** p < 0.001).

Article Snippet: Briefly, 2 μL RNA was used in a 20 μL PCR reaction system consisting of the Transcript Probe One-step qRT-PCR Super Mix kit (Tiangen, China) by using an Applied Biosystems 7500 Quantstudio 3,815 real-time quantitative PCR instrument (Life Technology, USA) under the following conditions: one cycle at 95°C for 5 min followed by 45 cycles at 94°C for 5 s and 60°C for 30 s. The viral RNA load in each sample was calculated based on Ct-values obtained from the constructed standard curves.

Techniques: Infection, Quantitative RT-PCR, Negative Control